Table 1. Primer and probe sequences, in 5′ to 3′ orientation, of LPV, HPyV6, HPyV7, WUV and KIV assays used in this study, along with their genomic target.
Oligo | Sequence | Virus | Target |
LPV-VP2-F | CATTGAAATAGAAGCAGTGGATCTTG | LPV | VP2 |
LPV-VP2-R | AAACTCCTATTCCTATGGCATTGTTG | ||
LPV-VP2-Prb | AGAGCAGTTTTCCCTCCTAAGTGCTATCCCAA | ||
HPyV6-VP2-F | TTGAGGAGCTGGACAAAGAGATT | HPyV6 | VP2 |
HPyV6-VP2-R | TCTGGGAAGCTTTTGAATTGGT | ||
HPyV6-VP2-Prb | AGGAAGATGCCTTGTCACAGAAAAGGAAATG | ||
HPyV6-LT-F | ACCAGGTGGGTGATGAAGACA | HPyV6 | LTAg |
HPyV6-LT-R | CGCCTGAATGTTTTAAAGGAGAA | ||
HPyV6-LT-Prb | TTGGTCCCTCAGGGTGGCATTCA | ||
HPyV7-LT-F | AAGACATTCAGTCTTTGCATTTTCTG | HPyV7 | LTAg |
HPyV7-LT-R | CCCCTCATACAGCATAAGGTTAGATT | ||
HPyV7-LT-Prb | CCACCTTTATCTGGATGATACTTTTTGCTGGC | ||
HPyV7-VP2-F | GAGGAAGGAAACACTCCCCAGTA | HPyV7 | VP2 |
HPyV7-VP2-R | TTCACTTCTTTTTGTAGCTCCTCAAG | ||
HPyV7-VP2-Prb | ACTATACCTCAATGGATGCTTTTTGT | ||
WUV-F-Reg-F | GCCGACAGCCGTTGGATATA | WUV | NCCR |
WUV-F-Reg-R | TTTCAGGCACAGCAAGCAAT | ||
WUV-F-Reg-Prb | AGGGTCACCATTTTTATTTCAGATGGGCA | ||
KIV-D-LT-F | CACAGGTGGTTTTCTATAAATTTTGTACTT | KIV | LTAg |
KIV-D-LT-R | GAATGCATACATCCCACTGCTTC | ||
KIV-D-LT-Prb | TGCATTGGCATTCGTGATTGTAGCCA | ||
KIV-E-Reg-F | GAACTTCTACTGTCCTTGACACAGGTA | KIV | NCCR |
KIV-E-Reg-R | GGATTAGAACTTACAGTCTTAGCATTTCAG | ||
KIV-E-Reg-Prb | TGGGAAACATCCGGTTTCCTCTCACTTCC | ||
MCV-LT1.1-F | AGCTCAGAAGTGACTTCTCTATGTTTGA | MCV | LTAg |
MCV-LT1.1-R | ACAATGCTGGCGAGACAACT | ||
MCV-LT1.1-Prb | TTTGCAGAGGTCCTGGGTGCATG |
LTAg = Large T Antigen, NCCR = Non Coding Control Region.