Figure 1. EYA is involved in innate immune responses against DNA.

mRNA levels were determined for antimicrobial peptide genes by quantitative RT-PCR and normalized to Rp49 expression(also known as RpL32). The relative values are indicated against Oregon-R as wild-type control. (A) Attacin A expression for DNaseII, eya ¹/+;DNaseII, eya ²/+;DNaseII, and eya^cli-IID/+;DNaseII mutant flies. One-way ANOVA was performed and followed by Dunnett's multiple comparison test. * indicates statistically significance (p<0.0001) by comparing to Oregon-R. (B) Attacin A expression for single eya, single DNaseII, and double DNaseII and eya knockdown flies at 8 days after heat-shock treatment. dsRNAs were expressed using the GAL4-UAS system. The GAL4 driver line alone was included. Two-tailed unpaired Student's t-test was performed for statistic analysis. (C) Drosomycin expression using the same sets of flies as for (B). (D) Attacin A expression by E.coli challenge for w¹¹¹⁸ as wild-type control and eya² mutant flies. The value represents the average and standard deviation of 3–6 independent experiments. A pool of 5–20 adult flies per genotype was collected for each experiment.