Figure 3. In vivo analysis of the role of the EYA threonine-phosphatase domain in Attacin A expression.

(A) Schematic views of eya rescue transgenic fly lines. Various EYA transgenes were introduced on the X chromosome by ΦC31 transgenesis. These flies carry eya² heterozygous and DNaseII^lo homozygous alleles on the 2^nd and the 3^rd chromosomes, respectively. The presence or absence of threonine and tyrosine phosphatase motifs in each transgene is shown by + or −. Bold lines in the transgenes are locations of replacement of amino acids. (B) Attacin A mRNA level was measured by quantitative RT-PCR and normalized to Rp49 expression(also known as RpL32). The relative values are indicated against Q355*. The value represents the average and standard deviation of three independent experiments. A pool of 5–7 adult flies per genotype was collected in each experiment. One-way ANOVA was performed (p = 0.0261) and followed by Dunnett's multiple comparison test. * indicates statistically significance (p<0.05) by comparing to WT.