Figure 4. Molecular basis for the interaction between compound 8 and heparin binding domain of VEGF₁₆₅.

A. Binding mode of compound 8 with heparin binding site of VEGF₁₆₅. The amino acid residues of heparin binding site of VEGF₁₆₅ are shown in stick models. B. Interactions of the compound 8 within the heparin binding pocket of VEGF₁₆₅ with putative hydrogen bonds shown as green dotted lines. The compound 8 is shown in green color. The bonding between the compound 8 and the heparin binding domain of VEGF₁₆₅ are shown in yellow color. All the hydrogen atoms are not shown. The picture is rendered in Discovery Studio, version 2.5 (right panel). C. Binding of VEGF₁₆₅ to immobilised-heparin. Various concentrations of compound 8 and VEGF₁₆₅ (250 nM) mixture in running buffer was injected onto the surface of the heparin-immobilised sensor chip. Sensograms obtained were overlaid using a BIA evaluation software (version 3.1).