Figure 8. Effect of the compound 8 against the metastatic potential of LM8G7 cells.

C3H/HeN mice were intravenously injected with LM8G7 cells via tail. Some mice received an intravenous injection of compound 8 (0.25, 0.5, or 1.0 mg/kg) on day 3, 5 and 10. After 4 weeks, the mice were sacrificed, the number of liver nodules was counted macroscopically, and the liver weight was measured in the control and compound 8-treated animals. Representative livers from mice injected with LM8G7 cells treated with DMEM (A), compound 8 (0.5 mg/kg) (B), and heparin (5 mg/kg) (C) are shown. The average liver weight of the mock and the compound 8 treated mice (D). Heparin from porcine intestinal mucosa was used as a positive control. The possible synergistic or additive effects of compound 8 and heparin were investigated by subsequent administration of compound 8 (0.25 mg/kg) and heparin (2.5 mg/kg) as described above. Data represent mean values ± SD for three independent experiments and each experiment was conducted with 6 mice per group. ^* P<0.05 versus control. Mann-Whitney U test.