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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Microbiology (Reading). 2009 Jun 25;155(Pt 10):3260–3269. doi: 10.1099/mic.0.028944-0

Fig. 1.

Fig. 1

The level of wzy expression affects OAg modal distribution in S. flexneri 2a. LPS profiles of wild-type and Δwzy strains grown in LB medium (lanes 1 and 2), and LPS profile of the Δwzy/pBAD-wzy strain grown under repressing (Glc) (lane 3) and inducing (Ara) conditions (lanes 4 and 5) to an OD600 of 1.0. LPS samples from equal numbers of bacterial cells were loaded in each lane and analysed by SDS-Tricine-PAGE on a 14 % acrylamide gel followed by silver staining. Strains are: 2457T (wild-type, wt), MSF114 (2457T Δwzy) and MSF114/pJC115 (2457T Δwzy/wzy).