. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: J Mol Neurosci. 2012 May 12;48(3):660–666. doi: 10.1007/s12031-012-9785-6

Table I.

Ability to inhibit the PACAP cytosolic Ca²⁺ response and PYK-2 tyrosine phosphorylation.

Addition	Ca²⁺ response	PYK-2 tyrosine phosphorylation
None	+	+
BAPTA, 1 μM	−	−
GF109203X, 5 μM	+	+/−
H89, 50 μM	+	+
PACAP(6–38), 10 μM	−	−
PP2, 1 μM	+	−
U73122, 50 μM	−	−

PACAP-27 (100 nM) was added to Fura2-AM loaded NCI-H838 cells and the Ca²⁺ response determined after 15 sec. PACAP-27 (100 nM) was added to NCI-H838 cells and the PYK-2 tyrosine phosphorylation determined after 2 min; +, stimulation; +/−, partial inhibition; −, complete inhibition. These experiments were repeated 4 times.