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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: J Immunol. 2009 Aug 12;183(5):3332–3343. doi: 10.4049/jimmunol.0900600

FIGURE 2.

FIGURE 2

Chemokine expression in response to C. rodentium infection. C57BL/6J mice were infected with C. rodentium or left uninfected (week 0), and mRNA expression was analyzed by real-time PCR for the indicated chemokines in the colon (A) or the indicated organs (B). Expression levels are shown relative to uninfected controls. Data are means ± SEM (n ≥ 4). C, Explants of whole colon tissue were cultured for 6 h, and levels of CXCL5 and CXCL9 in the supernatants were examined by ELISA. Data are means ± SEM (n ≥ 4). D, The indicated cell types were isolated from the colon of infected (2 wk) and uninfected mice by differential detachment (epithelial cells) or FACS (leukocyte subsets) and examined for CXC chemokine expression by real-time PCR. Data are shown as ratio of mRNA levels in cells from infected over uninfected mice. *, p < 0.05 by t test compared with the levels in uninfected mice.