FIG. 7.

Inhibition of PDGFR signaling. The addition of tyrphostin AG1295 (a selective inhibitor of PDGFR) to the medium significantly reduced the amount of αSMA+ cells near the vessels (A) and throughout the culture (B). (C) PDGF-B expression is significantly higher in hypoxia at day 14. The inhibition has insignificant effects on vessel length (D), and reduces vessel width in normoxia, but not hypoxia (E). Significance indicated vs. normoxia (*p<0.05, ***p<0.001) or vehicle (^#p<0.05, ^##p<0.01). Scale bar=200 μm. PDGF-B, platelet-derived growth factor-B; PDGFR, PDGF receptor. Color images available online at www.liebertonline.com/tea