FIG. 2.

Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) images of myotubes formed on cross-linked (PLL/HA) films. (A–C) Films were cross-linked with EDC10 (A), EDC70 (B), and EDC100 (C). Pictures were taken after 3 days in differentiation medium (DM) and captured at×4000 magnification, scale bar 30 μm. (D) CLSM sections on myotubes grown on EDC70 film for 5 days in DM (MHC in green and nuclei stain by Hoechst in blue). The bottom view represents a cross-section along the red line. Color images available online at www.liebertpub.com/tea