FIG. 4.

Early orientation of myoblasts after 15 h of culture in growth medium (GM) on the micropatterns. (A) Nuclei staining (Hoechst) and vinculin immunostaining of cells grown out of the micropatterns of increasing width, scale bar 50 μm. (B) Magnification of the nuclei of myoblasts on micropatterns, scale bar 20 μm. (C) Aspect ratio of nuclei (length over width of the equivalent ellipse) as a function of the width of the micropatterns. (D) Width of the nuclei. (E) Orientation of nuclei with regard to the main axis of the micropatterns (*p<0.05, **p<0.001). Color images available online at www.liebertpub.com/tea