FIG. 5.

Influence of polyelectrolyte multilayer (PEM)-coated micropatterns on myotube orientation after 5 days in differentiation medium. (A) Troponin T immunostaining and nuclear staining (Hoechst) highlighting the influence of the topography on myotubes alignment, scale bar 50 μm. (B) Angle distribution as a function of pattern width. Dashed red lines and values in red represent mean values for four independent experiments in 200–300 myotubes. Color images available online at www.liebertpub.com/tea