Figure 1.

Isolation and characterization of µ-CnIIIC. (A) Reverse-phase HPLC chromatogram (monitored by absorbance at 214 nm) of the venom duct extract from Conus consors. The peak eluting at ∼28 min contained µ-CnIIIC that was purified to homogeneity at an analytical scale and showed a single monoisotopic molecular mass (inset). The peptide was then subjected to reduction and mass spectrometry analysis. (B) ESI-MS/MS spectrum of the the m/z 596 undergoing collision-induced dissociation. The good fragmentation coverage allowed complete amino acid sequence assignment with y and b ions.