Figure 3.

Effect of µ-CnIIIC on the GAP recorded on isolated mouse sciatic and pike olfactory nerves. (A–B) Traces of GAP recorded in response to 0.05 ms (mouse) and 8 ms (pike) and 0.1–15 V stimulations, under control conditions and after treatment with µ-CnIIIC at the indicated concentrations. (C–D) GAP amplitude in response to different intensities of 0.05 and 8 ms stimulations and to different concentrations of µ-CnIIIC. (E–F) GAP amplitude in the presence of various concentrations of µ-CnIIIC, and expressed relative to control values as mean ± SEM of three to six mouse sciatic and three to four pike olfactory nerves. The curves were calculated from typical sigmoidal nonlinear regression through data points (r²≥ 0.984). The µ-CnIIIC concentrations required to block 50% of the GAP amplitude (IC₅₀) was 1.53 µM (mouse) and 0.15 µM (pike).