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. 2012 Jul 13;31(16):3494–3505. doi: 10.1038/emboj.2012.188

Figure 2.

Figure 2

Paf1C binds to phosphorylated Spt5 C-terminal repeats (CTR) peptides. (A–D) Peptide binding assays were conducted as described in Figure 1 using biotinylated CTR peptides (1.5 μg) with Ser1 unphosphorylated (CTR), Ser1 phosphorylated (pCTR), or Ser1 substituted with Asp (CTRD) or Glu (CTRE), and purified native Paf1C or recombinant GST fusion proteins, except using 1/5 or 1/10 input and supernatants of GST-Rtf1 and GST-Ctr9(594–855), respectively. Lanes 10–18 in (A) derive from the same experiment that produced Figure 1B, and the input and Mock lanes from the latter are reproduced here as lanes 10–12. Figure source data can be found with the Supplementary data.