Table 2.

Activity of the native, and recombinant SGalase 1 and SGalase 2 on β-(1→3)/(1→6)-D-linked polysaccharides and galacto-oligosaccharides

Substrate	Native enzyme		SGalase 1		SGalase 2
	Specific activity ± SD (μmol D-Gal/min/mg protein)	Relative specific activity (%)	Specific activity ± SD (μmol D-Gal/min/mg protein)	Relative specific activity (%)	Specific activity ± SD (μmol D-Gal/min/mg protein)	Relative specific activity (%)
Polysaccharides
β-(1→3)-D-Galactan (Acacia)	10.8 ± 0.3	100	12.4 ± 0.4f,c	100	3.8 ± 0.1f,c	100
dGA (Acacia)	4.4 ± 0.1	20	3.8 ± 0.2e,g	31	1.4 ± 0.1e,g	37
Gum arabic (Acacia)	0.03 ± 0.0	30	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
Arabinogalactan (Larch)	0.05 ± 0.0	30	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
β-(1→3;1→6)-D-Galactan (P. zopfii)	0.1 ± 0.0	1	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
Oligosaccharides
Methyl β-glycosides of β-(1→3)-D-
Galactobiopyranoside	63.2 ± 1.6	293	92.0 ± 5.8a,b	740	37.10 ± 1.9a,c	972
Galactotriopyranoside	nd	nd	28.5 ± 3.9d,b	229	5.2 ± 0.2e,c	137
Galactotetrapyranoside	49.8 ± 0.4	230	27.9 ± 1.1d,b	225	4.2 ± 0.1e,c	111
Galactopentaopyranoside	44.2	205	32.6 ± 6.0d,b	262	6.7 ± 0.1e,c	175
Galactoheptaopyranoside	nd	nd	21.7 ± 0.6d,b	175	3.4 ± 0.3e,c	89
Methyl β-glycosides of β-(1→6)-D-
Galactotriopyranoside	0.0 ± 0.0	0	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
Galactotetraopyranoside	nd	nd	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
Galactopentaopyranoside	0.2 ± 0.0	2	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0
Galactohexaopyranoside	0.2 ± 0.0	2	0.0 ± 0.0e,h	0	0.0 ± 0.0e,h	0

nd, not determined; Enzyme at ^a 0.1, ^d0.5, ^e0.7, ^f0.4 μg protein and incubated for ^b5, ^c15, ^g30 min, ^h24 h at 37 °C.