Figure 8.

Akt1 involves integrin α_vβ₃ in the modulation of transendothelial migration of prostate cancer cells. (A) Illustration of electric resistance by the endothelial monolayer measured as an index of rate of transendothelial migration by PC3 cells with the stable expression of either control vector (pBabe-Puro) or CA-Akt1 with the co-expression of either control vector (pcDNA3) or DN-integrin β₃ as determined by the ECIS equipment showing reduced endothelial resistance upon administration of CA-Akt1-expressing cells as compared with vector control expressing cells, which is inhibited by co-expression with DN-integrin β₃. (B) Bar graph showing quantification of the above data at 1 h time point. The data are presented as mean±s.d. (n=4 of quadruplicate experiments); *P<0.001 and ^#P<0.05.