Table 6. Analysis of mRNA expression levels and of lipid peroxidation in the remote LV myocardium in non-infarcted control C57BL/6 LDLr−/− mice and in control and AdLDLr-treated C57BL/6 LDLr−/− mice 28 days after MI.
| Non-infarcted |
28 Days after MI |
||
|---|---|---|---|
| Control | Control | AdLDLr | |
| Gene expression (mRNA expression normalized to Gapdh) | |||
| Agtr1a | 1.0±0.098 | 0.43±0.018§§§ | 0.58±0.033***§§ |
| Ctgf | 1.0±0.15 | 9.3±1.1§§§§ | 5.2±0.96*§§§ |
| Nox2 | 1.0±0.12 | 2.0±0.21§§ | 1.5±0.22 |
| Nox4 | 1.0±0.19 | 9.0±1.2§§§§ | 4.8±0.95*§§§ |
| Nppa | 1.0±0.29 | 10±1.3§§§§ | 11±3.1§§§ |
| Nppb | 1.0±0.22 | 4.8±0.31§§§§ | 2.6±0.42***§§ |
| Sod1 | 1.0±0.10 | 1.2±0.070 | 1.0±0.12 |
| Sod2 | 1.0±0.10 | 0.96±0.078 | 1.0±0.079 |
| Sod3 | 1.0±0.31 | 1.7±0.20 | 1.6±0.31 |
| Lipid peroxidation | |||
| MDA (nmol mg protein–1) | 2.5±0.19 | 2.2±0.17 | 2.7±0.17 |
Abbreviations: AdLDLr, adenoviral LDLr; Agtr1a, angiotensin II receptor, type 1a; Ctgf, connective tissue growth factor; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; MDA, malondialdehyde; MI, myocardial infarction; Nox, NADPH oxidase; Nppa, natriuretic peptide precursor type A; Nppb, natriuretic peptide precursor type B; LDLr, LDL receptor; LV, left ventricular; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction Sod, superoxide dismutase; TBARS, thiobarbituric acid reactive substances.
*P<0.05, ***P<0.001 for comparison of control versus AdLDLr-treated mice.
§§P<0.01, §§§P<0.001, §§§§P<0.0001 for comparison versus non-infarcted mice.
Data are presented as means±s.e.m. qRT-PCR analysis (n=7–15 per group) was performed using Taqman gene expression assays. Gene expression data analysis was performed using ΔΔCt-based fold-change calculations and data are expressed relative to values in non-infarcted control mice. The degree of lipid peroxidation in LV homogenates (n=8 per group) is expressed as MDA equivalents (nmol) per mg protein using the TBARS assay according to the instructions of the manufacturer (Cayman Chemical).