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. 2011 Oct 11;36(8):1032–1039. doi: 10.1038/ijo.2011.193

Figure 2.

Figure 2

The involvement of proteins in different signaling pathways on the preadipocyte proliferation induced by rSAA. (a) Pretreatment of 3T3-L1 cells with pertussis toxin under the conditions described in the methods. (b) 3T3-L1 preadipocytes were pretreated with SB 203580, PD98059 and wortmannin and then stimulated with rSAA. (c) The influence of insulin (100 nM) and rSAA (5 μg ml−1) on activation of the PI3 K pathway. Data are the mean±s.e. of three independent experiments, which were each performed in triplicate and one-way analysis of variance was performed in A and two-way analysis of variance was performed in B and C (**P<0.01, ***, φφφP<0.001 vs control groups).