Figure 8.

Serine phosphorylation of occludin, zonula occludens-1 in the terminal ileum. Tissue lysates were subjected to immunoprecipitation with the anti-occludin or zonula occludens-1 (ZO-1) antibody, followed by Western blotting analysis with antibodies against phosphoserine. A: p-Occludin and p-ZO-1 protein levels compared with untreated cells; B: Effects of addition of Lactobacillus plantarum on the expression of the p-occludin and p-ZO-1 proteins as shown by relative band density. Data in the bar graph represent mean ± SD of the three separate experiments. ^aP < 0.05 Group II vs Group III; ^cP < 0.05 Group IV vs Group V. Group I : Sham-operation; Group II: Bile duct ligation (BDL); Group III: BDL + Lactobacillus plantarum (L. plantarum); Group IV: BDL + internal biliary drainage (IBD); Group V: BDL + IBD + L. plantarum.