Table 1.
Sequences of oligonucleotide primers and conditions for real-time polymerase chain reaction
| Gene target | Genbank number (mRNA) | Oligonucleotide1 (5'- to 3'-) | Annealing temperature (°C) | Product size (bp) |
| Occludin | NM-031329 | F: GCTCAGGGAATATCCACCTATC R: TTCTCCAGCAACCAGCATC | 60 | 344 |
| ZO-1 | NM-001106266 | F: CCACAGACATCCAACCAGC R: AGCCCAAAGAACAGAAGACC | 60 | 247 |
| Claudin-1 | NM-031699 | F: GCCTCCAATGCCGTTCT R: TGCCTGCGTCCCTCTTG | 60 | 317 |
| Claudin-4 | NM-001012022 | F: GTTCCCGCCAGCAACTATG R: CCTTCAGCCCCGTATCCA | 60 | 282 |
| JAM-A | NM-053796 | F: CCTCCATCCAAGCCGACA R: CAAAGACCAAATCCCCTGAC | 60 | 211 |
| prkC | NM-001105713 | F: GATGGACGGGGTCACGA R: CGCTTGGCAGGGTGTTT | 60 | 165 |
| β-actin | NM-031144 | F: CAGGTCATCACTATCGGCAAT R: GAGGTCTTTACGGATGTCAAC | 60 | 144 |
1
Primers were designed based on sequences of rat-corresponding genes from the GenBank database. JAM-A: Junction adhesion molecule-A; ZO-1: Zonula occludens-1.