Figure 5.
Selective Depletion of Germ Cell Transcripts by JQ1
(A) Heatmap representation of the top 25 down- and upregulated genes (Q < 0.05) following treatment of male mice with JQ1 (50 mg/kg from 6–12 weeks). Plk1 and Ccna1 (bold) are downregulated by JQ1 exposure.
(B) GSEA of three spermatogenesis gene clusters (Schultz et al., 2003) in the testicular transcriptional profile of JQ1-treated males.
(C) GSEA of a canonical MYC-dependent gene set (Zeller et al., 2003) in the testicular transcriptional profile of JQ1-treated male mice.
(D) Quantitative RT-PCR analysis of males treated from 6–12 weeks of age with JQ1 or vehicle. The mouse genes are Plzf (promyelocytic leukemia zinc-finger or Zbtb16), Stra8 (stimulated by retinoic acid gene 8), Brdt (bromodomain, testis-specific), Ccna1, Hist1h1t (histone cluster 1, histone 1, testis-specific), Msy2 (Y box protein 2 or Ybx2), Papolb (poly (A) polymerase β or Tpap), Klf17 (Kruppel-like factor 17 or Zfp393), and Prm1 (protamine 1). Data represent the mean ± SEM and are annotated with p values as obtained from a two-tailed t test (∗p < 0.05; ∗∗p < 0.001; the p value for Prm1 is 0.06).