Figure S1.

Motility and Histological Effect of JQ1 Treatment on the Epididymides of Male Mice, Related to Figure 3

(A) Progressive motility of mature sperm obtained from the epididymis of males treated with JQ1 (50 mg/kg QD) or control from 6-12 weeks of age. Data was obtained using video analysis of sperm on a 37°C heated stage.

(B) Treatment of male mice with JQ1 (50 mg/kg QD) for 8 weeks does not affect the mass of seminal vesicles.

(C) Histology of the epididymides from male mice treated with control or JQ1 (50 mg/kg QD) from 6-12 weeks of age. Fewer spermatozoa and multiple large nucleated cells (black arrow) are observed in the epididymal lumen of the JQ1-treated mice compared to the control epididymal lumen, which is densely packed with mature spermatozoa.