Figure S4.

Reversal of Testicular Phenotypes following Withdrawal of JQ1, Related to Figure 7

(A–C) Analysis of (A) testicular mass, (B) sperm counts, and (C) sperm motility of adult male mice treated for 3 weeks with JQ1 (50 mg/kg QD), followed by euthanasia at the end of treatment (JQ1), 2 months following cessation of treatment, and 4 months following cessation of treatment. Normalization of all parameters is evident following drug withdrawal. Data represent mean ± SEM.

(D and E) Testis histology (10X) of tubules from control and JQ1-treated male mice 4 months following cessation of JQ1 as treated in (A-C).

(F) Graphical representation of tubule area calculated from histological preparations of testis tubules from male mice following four month recovery from the low-dose (Figure 6A) regimen. Data represent mean ± SEM.

(G) Graphical representation of tubule area calculated from histological preparations of testis tubules from male mice during and following 10 day, 30 day, and 60 day recovery from a 3 week period of JQ1 BID treatment. Data represent mean ± SEM (^∗, significant at p < 0.05).

(H) A single pup born from a JQ1-treated male after halting JQ1 treatment. Normal developmental and behavioral phenotypes are observed in offspring of mice with restored fertility following a period of contraception conferred by JQ1 treatment.