Figure S5.

JQ1 Reduces Testis Size, Sperm Counts, and Motility in Male Rats, Accompanied by Histological Evidence of Impaired Spermiogenesis, Related to Figure 4

(A) Pharmacokinetic analysis of JQ1 in the plasma of male rats following a single administration of JQ1 (50 mg/kg IP). Data represent the mean ± SD.

(B–D) Graphical representation of (B) testis mass, (C) sperm count and (D) sperm motility derived from adult male Sprague-Dawley rats treated with intraperitoneal JQ1 for 3 weeks, as described in the Experimental Procedures. Reduction of each parameter is consistent with an anti-spermatogenic effect of BRDT bromodomain inhibition by JQ1. Data represent the mean ± SEM (^∗, significant at p < 0.05).

(E and F) Testis histology (40x) of seminiferous tubules from control and JQ1-treated male rats. Comparable depletion of maturing spermatocytes and round spermatids is evident in treated Sprague-Dawley rats compared to male mice (as shown in Figure 4).