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. 2012 Aug 16;4(5):e00095. doi: 10.1042/AN20120022

Figure 2. D-Asp-induced mTOR phosphorylation is GLAST activity-dependent.

Figure 2

In all panels phosphorylated mTOR was detected as described for Figure 1. (A) MGC monolayers were incubated for 15 min with Glu (1 mM), THA (100 μM), the AMPA receptor antagonist CNQX (50 μM), and CNQX plus THA, in this case CNQX was added 30 min prior to THA treatment (100 μM, 15 min). MGC cultures were pre-exposed to NMDA receptor antagonists MK-801 (5 μM) or LAP5 (10 μM) (B) and to metabotropic Glu receptors antagonists, for group I CPCCOEt (100 μM) and for group II/III CPPG (300 μM) (C), 30 min prior to D-Asp treatment (1 mM, 15 min). Data are expressed as the mean ±S.E. of at least three independent experiments. Representative Western blots are shown for each condition. Statistical analysis was performed comparing against non-stimulated cells using a non-parametric one-way ANOVA (Kruskal–Wallis test) and Dunn's post-hoc test (*P<0.05, ***P<0.001).