Figure 7. GLAST activity signals to AP-1 DNA binding.

(A) EMSAs. Nuclear extracts were prepared from control or treated cells (Glu, D-Asp, THA or TBOA) and binding to the AP-1 consensus sequence was analysed as described in the Materials and methods section. (B) Nuclear extracts were prepared from cells pre-incubated 30 min with Na⁺/Ca²⁺ exchanger inhibitor (KB-R7943, 15 μM) and with the calmodulin antagonist (W7, 25 μM), after that treated with D-Asp (1 mM, 90 min) the binding to the AP-1 consensus sequence was analysed. The results are the mean ±S.E. of at least three independent experiments. Statistical analysis was performed comparing against non-stimulated cells using a non-parametric one-way ANOVA (Kruskal–Wallis test) and Dunn's post-hoc test (*P<0.05, ***P<0.001).