Table 2.
Levels of cytochromes c produced under aerobic and anaerobic conditions in a set of four bacterial strains. The levels for cytochrome c550 were determined spectroscopically whereas for cytochromes cd1, NapB and NrfA SDS–PAGE analysis followed by densitometry on heme-stained gels was used. At least 6 replicates were done for each experiment and errors for each dataset are given in brackets. The amount of cytochrome produced by the wild-type strain is arbitrarily set to 100.
|
Aerobic conditions | |||
|---|---|---|---|
| Cytochrome c550 |
Cytochrome cd1 |
||
| Strain | Level of cytochrome c | Strain | Level of cytochrome c |
| MC1000 | 100 (±9) | MC1000 | 100 (±13) |
| ΔdsbA | 94 (±2) | ΔdsbA | 100 (±13) |
| ΔdsbD | 6 (±6) | ΔdsbD | 29 (±10) |
| ΔdsbA/ΔdsbD | 53 (±12) | ΔdsbA/ΔdsbD | 54 (±19) |
| Anaerobic conditions | |||
| Cytochrome c550 | Cytochrome cd1 | ||
| Strain | Level of cytochrome c | Strain | Level of cytochrome c |
| MC1000 | 100 (±8) | MC1000 | 100 (±8) |
| ΔdsbA | 30 (±0) | ΔdsbA | 39 (±8) |
| ΔdsbD | 10 (±5) | ΔdsbD | 23 (±4) |
| ΔdsbA/ΔdsbD | 21 (±19) | ΔdsbA/ΔdsbD | 31 (±10) |
| Cytochrome NapB | Cytochrome NrfA | ||
| Strain | Level of cytochrome c | Strain | Level of cytochrome c |
| MC1000 | 100 (±4) | MC1000 | 100 (±2) |
| ΔdsbA | 44 (±7) | ΔdsbA | 17 (±6) |
| ΔdsbD | 4 (±13) | ΔdsbD | 6 (±3) |
| ΔdsbA/ΔdsbD | 39 (±1) | ΔdsbA/ΔdsbD | 13 (±2) |