Table 1.

Selected characteristics of prepared NPs: (i) efficiency of PLL and PEG-PLL coating on BSA NPs, (ii) ζ-potential, and (iii) average size (average ± standard deviation, n ≥ 5). Note that the NPs from 4.2 kDa PLL-PEG coating gave two size populations with relative ratios of 14% and 86%.

	Coated amount (mg conjugate/mg BSA)	ζ-Potential  (mV)	Diameter  (nm)
Uncoated	—	−10.1 ± 0.4	255 ± 30
4.2 kDa PLL	0.12 ± 0.03	10.1 ± 0.5	350 ± 90
24 kDa PLL	0.10 ± 0.02	20.4 ± 0.5	310 ± 30
4.2 kDa PEG-PLL	0.016 ± 0.006	1.8 ± 0.3	220 ± 26  (14%)
			880 ± 100  (86%)
24 kDa PEG-PLL	0.007 ± 0.003	7.8 ± 0.9	845 ± 140