Fig. 2.

Tlr4^−/− CD4⁺ T cells exhibit blunted effector cytokine responses in vivo. (A) Representative staining of CD4⁺ T cells derived from the DLN of WT- (Left) or Tlr4^−/−-transferred (Right) Rag1^−/− mice immunized 7 d with KLH. Cells were restimulated overnight with KLH before intracellular cytokine staining. (B) Summary of CD4⁺ T-cell cytokine production in the DLN following KLH immunization. (C) Proliferation analysis of WT and Tlr4^−/− CD4⁺-transferred DLN following 7 d KLH immunization. (D) Cytokine production analysis of splenic (Top) and DLN (Bottom) CD4⁺ T cells following 3 d KLH restimulation. For all experiments, n = 5 per group; *P < 0.05; Student's t test. Data are representative of three independent experiments.