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. 2012 Jul 23;109(32):13064–13069. doi: 10.1073/pnas.1120585109

Fig. 4.

Fig. 4.

TLR4-deficient CD4+ T cells lack encephalitogenic cytokine production. (A) Representative CD4 and CD45.1 staining in the CNS (Left) and spleen (Right) of B6SJL:Tlr4−/− chimeric mice following EAE induction. (B) Representative cytokine staining of WT (CD45.1) and Tlr4−/− (CD45.2) CD4+ T cells in the CNS following EAE induction and PMA restimulation. (C) Summary of CD4+ T-cell frequencies in the CNS of mixed bone marrow–reconstituted mice following EAE induction. n = 5 mice per group. (D) Intracellular cytokine staining of splenocytes from the mice in A following overnight MOG35–55 restimulation. Each column (Top and Bottom) is representative of the CD45.1 and CD45.2 CD4+ T cells derived from one individual mouse. (E) Compilation of the data presented in D. (F) Cytokine production analysis of splenic CD45.1 or CD45.2 CD4+ T cells that were cultured 3 d with APCs in the absence or presence of MOG35–55 peptide. Data are representative of three independent experiments. *P < 0.05; Student's t test.