Fig. 1.

Migration of MDCK cell sheet on fibronectin strips of different widths. (A) Schematic of the fibronectin stamped pattern with a block of PDMS (gray). Cells reach confluence in the reservoir (shown as a yellow area) and migrate into the strips when the PDMS block is lifted (as illustrated by the last step). (B) MDCK cell sheets migrating on fibronectin strips of different widths. (C) Average displacement of cell front over time in 400, 100, and 20-μm wide strips. (D) Velocity of cell front on strips of different widths for untreated (black) and blebbistatin-treated (red) MDCK cells. Dashed lines are a smooth fit to guide the eye. (Scale bar, 100 μm).