Fig. 2.

(A) Analysis of growth in LB broth of Salmonella wild-type strain ST14028s and vector strain SL201 and its derivatives that carry constructs pU6-mPR1, pU6-mPR2, and pU6-TK1. (B and C) Northern analyses of the EGS RNA expression in J774 macrophages treated with strain SL201 carrying the empty vector pU6 (-, lane 1) and pU6-mPR1 (lane 3 in B and C) or with strain SL7207 carrying pU6-mPR1 (lane 2 in B and C). RNA samples (25 μg) were separated on 2% agarose gels that contained formaldehyde, transferred to nitrocellulose membranes, and hybridized to a [³²P]-radiolabeled probe that contained the DNA sequence coding for mPR1 RNA (B) or mouse RNase P RNA (C).