Fig. 4.
High-resolution AFM images of tall (A, C) and short (B, D) domains. A and B show unprocessed AFM images. C and D show high resolution height images of four tetramers from tall and short domains that reveal distinct topological differences C and D were processed through correlation averaging (see SI Appendix for details). S-layers were initially grown in protein solution with Cp ∼ 41 μg/mL (10 mM Tris pH 7.2, 50 mM CaCl2, 100 mM NaCl) at 25 °C for 4.5 h. Both A and B were imaged in the same solution with minimal imaging forces (ca. 85 pN) (full Z color scale: 8 nm for A and B, 2.5 nm for C and D).