Fig. 3.

Reduction in GA signaling results in a decrease in SHR-GFP in the endodermis. (A and B) Short-term treatment of 5-d-old roots with PAC (B) resulted in a significant increase in the expression of pSHR-erGFP (quantified in Fig. S3C) that did not translate into an increase in the amount of SHR-GFP protein as indicated by similar levels of SHR-GFP in the stele of untreated and PAC-treated roots. (C and D, also see Fig. S3D) However, there was a significant decrease in the endodermal-to-stele ratio of SHR-GFP after PAC treatment (D), indicating an overall decrease in the levels of SHR-GFP in the endodermis.