Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Mar;5(3):951–960. doi: 10.1093/nar/5.3.951

Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.

U Kuhnlein, B Lee, E E Penhoet, S Linn
PMCID: PMC342035  PMID: 643622

Abstract

Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.

Full text

PDF
952

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cleaver J. E., Bootsma D. Xeroderma pigmentosum: biochemical and genetic characteristics. Annu Rev Genet. 1975;9:19–38. doi: 10.1146/annurev.ge.09.120175.000315. [DOI] [PubMed] [Google Scholar]
  2. Cleaver J. E. Defective repair replication of DNA in xeroderma pigmentosum. Nature. 1968 May 18;218(5142):652–656. doi: 10.1038/218652a0. [DOI] [PubMed] [Google Scholar]
  3. Day R. S., 3rd Studies on repair of adenovirus 2 by human fibroblasts using normal, xeroderma pigmentosum, and xeroderma pigmentosum heterozygous strains. Cancer Res. 1974 Aug;34(8):1965–1970. [PubMed] [Google Scholar]
  4. Fornace A. J., Jr, Kohn K. W., Kann H. E., Jr DNA single-strand breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum. Proc Natl Acad Sci U S A. 1976 Jan;73(1):39–43. doi: 10.1073/pnas.73.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goth-Goldstein R. Repair of DNA damaged by alkylating carcinogens is defective in xeroderma pigmentosum-derived fibroblasts. Nature. 1977 May 5;267(5606):81–82. doi: 10.1038/267081a0. [DOI] [PubMed] [Google Scholar]
  6. Kirtikar D. M., Dipple A., Goldthwait D. A. Endonuclease II of Escherichia coli: DNA reacted with 7-bromomethyl-12-methylbenz[alpha]anthracene as a substrate. Biochemistry. 1975 Dec 30;14(26):5548–5553. doi: 10.1021/bi00697a002. [DOI] [PubMed] [Google Scholar]
  7. Kirtikar D. M., Goldthwait D. A. The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea. Proc Natl Acad Sci U S A. 1974 May;71(5):2022–2026. doi: 10.1073/pnas.71.5.2022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kraemer K. H., Coon H. G., Petinga R. A., Barrett S. F., Rahe A. E., Robbins J. H. National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014, USA. Proc Natl Acad Sci U S A. 1975 Jan;72(1):59–63. doi: 10.1073/pnas.72.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kraemer K. H., De Weerd-Kastelein E. A., Robbins J. H., Keijzer W., Barrett S. F., Petinga R. A., Bootsma D. Five complementation groups in xeroderma pigmentosum. Mutat Res. 1975 Dec;33(2-3):327–340. doi: 10.1016/0027-5107(75)90208-0. [DOI] [PubMed] [Google Scholar]
  10. Kuhnlein U., Penhoet E. E., Linn S. An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169–1173. doi: 10.1073/pnas.73.4.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lindahl T. New class of enzymes acting on damaged DNA. Nature. 1976 Jan 1;259(5538):64–66. doi: 10.1038/259064a0. [DOI] [PubMed] [Google Scholar]
  12. Lindahl T., Nyberg B. Heat-induced deamination of cytosine residues in deoxyribonucleic acid. Biochemistry. 1974 Jul 30;13(16):3405–3410. doi: 10.1021/bi00713a035. [DOI] [PubMed] [Google Scholar]
  13. Lindahl T., Nyberg B. Rate of depurination of native deoxyribonucleic acid. Biochemistry. 1972 Sep 12;11(19):3610–3618. doi: 10.1021/bi00769a018. [DOI] [PubMed] [Google Scholar]
  14. Russell W. C., Newman C., Williamson D. H. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  15. Schneider E. L., Stanbridge E. J., Epstein C. J. Incorporation of 3H-uridine and 3H-uracil into RNA: a simple technique for the detection of mycoplasma contamination of cultured cells. Exp Cell Res. 1974 Mar 15;84(1):311–318. doi: 10.1016/0014-4827(74)90411-x. [DOI] [PubMed] [Google Scholar]
  16. Shapiro R., Klein R. S. The deamination of cytidine and cytosine by acidic buffer solutions. Mutagenic implications. Biochemistry. 1966 Jul;5(7):2358–2362. doi: 10.1021/bi00871a026. [DOI] [PubMed] [Google Scholar]
  17. Sutherland B. M., Oliver R., Fuselier C. O., Sutherland J. C. Photoreactivation of pyrimidine dimers in the DNA of normal and xeroderma pigmentosum cells. Biochemistry. 1976 Jan 27;15(2):402–406. doi: 10.1021/bi00647a025. [DOI] [PubMed] [Google Scholar]
  18. Sutherland B. M., Rice M., Wagner E. K. Xeroderma pigmentosum cells contain low levels of photoreactivating enzyme. Proc Natl Acad Sci U S A. 1975 Jan;72(1):103–107. doi: 10.1073/pnas.72.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tanaka K., Sekiguchi M., Okada Y. Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus). Proc Natl Acad Sci U S A. 1975 Oct;72(10):4071–4075. doi: 10.1073/pnas.72.10.4071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wolff S., Rodin B., Cleaver J. E. Sister chromatid exchanges induced by mutagenic carcinogens in normal and xeroderma pigmentosum cells. Nature. 1977 Jan 27;265(5592):347–349. doi: 10.1038/265347a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES