FIG. 2.
The KD values and titers of anti-OVA MAbs determined by ELISA and RD-ELISA. (A) KD values of (•)[solid circle] no. 20 and (▪)[solid square] no. 24 were 2.6×10−8 M and 3.3×10−9 M, respectively, calculated by Eq 1, in which A is the absorbance measured for the antibody reacting with different concentrations of antigen, A0 is the absorbance measured for the antibody in the absence of antigen, and a0 is the total antigen concentration. (B) The titers of (•)[solid circle] no. 20 and (▪)[solid square] no. 24 were 6.3×10−4 and 3.9×10−5. The points are given as mean±SD values of triplicate determinations.