Table 1.
Characteristics of Anti-OVA MAbs: Indirect ELISA Titer, Affinity, and RD-ELISA Titer
| Clone | Subclass | Titer detected by indirect ELISA | Affinity detected by ELISA | Titer detected by RD-ELISA |
|---|---|---|---|---|
| 25 | IgG1(κ) | 5.0×10−7 | 1.9×10−9 | 7.8×10−5 |
| 4 | IgG1(κ) | 3.1×10−7 | 2.8×10−9 | 3.1×10−5 |
| 20 | IgG1(κ) | 2.5×10−7 | 2.6×10−8 | 6.3×10−4 |
| 1 | IgG1(κ) | 2.5×10−7 | 9.5×10−9 | 3.1×10−4 |
| 3 | IgG1(κ) | 2.5×10−7 | 3.5×10−9 | 3.9×10−5 |
| 22 | IgG1(κ) | 2.0×10−7 | 2.1×10−8 | 6.3×10−4 |
| 24 | IgG1(κ) | 2.0×10−7 | 3.3×10−9 | 3.9×10−5 |
| 27 | IgG1(κ) | 1.6×10−7 | 2.7×10−8 | 6.3×10−4 |
| 23 | IgG1(κ) | 1.0×10−7 | 2.7×10−9 | 3.9×10−5 |
| 19 | IgG1(κ) | 6.3×10−8 | 5.6×10−9 | 3.1×10−4 |
| 21 | IgG1(κ) | 6.3×10−8 | 4.8×10−9 | 3.1×10−4 |
| 9 | IgG1(κ) | 5.0×10−8 | 8.9×10−9 | 6.3×10−4 |
| 12 | IgG1(κ) | 5.0×10−8 | 8.1×10−9 | 3.1×10−4 |
| 13 | IgG1(κ) | 5.0×10−8 | 1.1×10−9 | 7.8×10−5 |
| 7 | IgG1(κ) | 2.5×10−8 | 7.8×10−10 | 3.9×10−5 |
| 17 | IgG1(κ) | 5.0×10−9 | 2.8×10−8 | 6.3×10−4 |
| 14 | IgG1(κ) | 5.0×10−9 | 6.2×10−10 | 3.9×10−5 |
| 6 | IgG1(κ) | 3.1×10−9 | 8.7×10−10 | 3.9×10−5 |
| 5 | IgG2b(κ) | 2.5×10−9 | 3.6×10−8 | 6.3×10−4 |
| 10 | IgG1(κ) | 1.6×10−9 | 6.2×10−10 | 3.9×10−5 |
| 2 | IgG2b(κ) | 1.0×10−3 | ND | ND |
| 8 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 11 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 15 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 16 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 18 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 26 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 28 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 29 | IgG1(κ) | 1.0×10−3 | ND | ND |
| 30 | IgG1(κ) | 1.0×10−3 | ND | ND |
Titers of 30 MAbs were tested by indirect ELISA, and 20 MAbs with a titer higher than 1×10−3 were selected for the subsequent affinity and RD-ELISA titer detection.
ND, not detected.