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. 1978 Apr;5(4):1139–1152. doi: 10.1093/nar/5.4.1139

A new method for the purification and identification of covalently closed circular DNA molcules.

M Zasloff, G D Ginder, G Felsenfeld
PMCID: PMC342066  PMID: 26042

Abstract

A new technique has been developed for the rapid isolation of covalently closed circular DNA molecules. The procedure is a selective extraction based on differences in the partitioning of covalently closed circular DNA molecules and noncovalently closed species between phenol and water at acid pH and low ionic strength. Under the conditions described, linear as well as nicked circular DNA is extracted into phenol, while covalently closed circular DNA molecules remain in the water phase. The method permits the quantitative isolation of covalently closed circular DNA from either total cellular DNA or partially purified preparations, to a degree of purity comparable with buoyant density procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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