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Analysis of TuD-directed inhibition of miR-122 in HuH-7 cells. (a) Anti-miR-122 TuDs used. (b) Dose-response curves for inhibition of miR-122 function in HuH-7 cells. Cells were transfected with constructs expressing the TuDs, together with a psiCHECK-2 reporter plasmid bearing three sites complementary to miR-122. Values are mean ± s.d. (n = 3). (c) Northern hybridization analysis of the experiment in (b).
