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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Exp Cell Res. 2010 Mar 19;316(10):1706–1715. doi: 10.1016/j.yexcr.2010.03.005

Figure 2. 12-LOX upregulates HIF-1α mRNA in prostate cancer cells under hypoxia.

Figure 2

Northern blotting was performed to measure the abundance of HIF-1α message in nL-8 and neo cells as outlined in “materials and methods”, using total mRNA isolated from nL-8 and neo cells incubated under hypoxic conditions for 6 h. Shown here is a representative result from two independent experiments.