. Author manuscript; available in PMC: 2012 Aug 16.

Published in final edited form as: Exp Cell Res. 2010 Mar 19;316(10):1706–1715. doi: 10.1016/j.yexcr.2010.03.005

Table 1. Real-time PCR analysis of HIF-1α transcript levels in nL-8 and neo cells cultured under hypoxic conditions.

The relative abundance of the message was calculated using the ΔCT method as explained previously [8]. Shown here is representative data from three independent experiments performed under identical conditions.

Cell type	ΔC_T	ΔΔC_T	T test	nL-8:neo
nL-8	5.84	−1.48	P = 0.013*	2.79:1
neo-α	7.32	−1.48	P = 0.013*	2.79:1