Figure 8. Jak2 V617F G935R displays a strong degree of inhibitor resistance.

293T cells were co-transfected with the TEL-JAK2 construct indicated and a GST-JAK2 substrate fusion gene (GST-J2s-KEYY). Post-transfection, cells were incubated with the indicated JAK Inhibitor-I concentration for four hours. Cells were lysed and the GST fusion protein was isolated. Phosphorylation of the JAK2 substrate and the Jak2 V617F mutant protein were examined by the phospho-tyrosine specific antibody 4G10. Total GST and total Jak2 were also assessed. The figure is representative of three independent experiments.