Figure 6. Rho GTPase expression during DC maturation.

(A) Rho GTPase expression in DCs during maturation with LPS or PGE₂. The percentage of total Rho GTPase expression is depicted for each DC in a pie chart. Rho GTPase subfamilies and individual Rho GTPases are color coded. iDCs; immature DCs, mDCs (LPS); LPS-matured DCs, mDCs (PGE2); PGE₂-matured DCs. (B) The 2^−ΔΔCt values of the Rho GTPases in immature and mature DCs. The 2^−ΔΔCt values of the individual data points for each cell type are depicted. Donormix 1 and 2 are derived from 9 and 3 donors, resp. Donormix CD14 is derived from the same donors as donormix 2, but monocytes were obtained by elutriation followed by CD14 MACS isolation and differentiated to DCs. iDCs; immature DCs, mDCs (LPS); LPS-matured DCs, mDCs (PGE2); PGE₂-matured DCs.