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. 2012 Aug 16;7(8):e43008. doi: 10.1371/journal.pone.0043008

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) RT-PCR of sorted cell fractions with minimal (CCSP⁻ fraction) and strong (CCSP⁺ fraction) expression of CCSP mRNA. B–E) Immunofluorescence staining of sorted cell fractions. B) CCSP (green fluorescence) was expressed in almost all cells of the CCSP⁺ fraction, but rarely in the CCSP⁻ fraction. C) Many cells were also positive for the Clara cell marker UGRP1 (green fluorescence) in CCSP⁺ fraction cells. D) Pan-keratin expression (red fluorescence) in CCSP⁺ cells confirmed that they were epithelial. Only small population of CCSP⁻ cells expressed pan-keratin. E) Few cells in either fraction expressed pro-SPC (red fluorescence). Nuclei appear blue (DAPI); Bar = 20 µm; CCSP Sorting negative = CCSP⁻ fraction; CCSP Sorting positive = CCSP⁺ fraction.