Figure 5. Bronchosphere formation by sorted cells.

A) Phase-contrast photomicrographs of bronchospheres (Bar = 100 µm). B) The diameter of all spheroid colonies in the wells which 5000 cells were seeded. Bar graph reveals average spheroid colony size (µm). (Mean ±S.D. p = 0.0048, Student t-test). C) Average spheroid colony number per well in serial diluted cells after one week sphere cell culture. D) Efficiency of sphere colony formation = total colony number/total seeded cell ×1000. Efficiency of sphere colony formation was higher in CCSP⁻ cells than that in CCSP⁺ cells (p<0.0001, Chi-square test).