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. 2012 Aug 16;8(8):e1002892. doi: 10.1371/journal.pgen.1002892

Figure 1. hUTP4/Cirhin interacts with NOL11.

Figure 1

(A) hUTP4/Cirhin was screened against a human liver cDNA library using the yeast two-hybrid methodology. Two positive clones were recovered (clones 5 and 6), both of which were C-terminal fragments of nucleolar protein 11 (NOL11). (B) Directed Y2H analysis confirms that hUTP4/Cirhin interacts with the two positive clones as well as with full-length NOL11 (NOL11-FL). EV is the empty Y2H prey vector. Growth of a blue yeast colony indicates interaction. (C) NOL11 is conserved throughout metazoans. BLAST searches using human NOL11 (NP_056277.2) as a query were used to identify NOL11 orthologs in other species. BioEdit [57] was used to calculate percent identity and similarity.