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. 2012 Aug 16;8(8):e1002881. doi: 10.1371/journal.pgen.1002881

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© 2012 Nakdimon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Total extracts of wild-type, daf-18(lf), let-60(gf) and daf-18(lf) let-60(gf) larvae at the L4 stage were analyzed on Western blots using antibodies against (A) phosphorylated and total MEK-2 and (C) against di-phosphorylated (dp) and total MPK-1. Signal intensities were quantified in four (for MEK-2) and three (for MPK-1) independent experiments, and the average ratios of (B) pMEK-2 to total MEK-2 and (D) dpMPK-1 to total MPK-1 intensities were calculated.