Table 3. Epistasis analysis of daf-18 with components of the RAS/MAPK pathway.

	genotype	Induction±SE	% Vul	% Muv	n
1	wild-type	3.00±0.00	0.0	0.0	many
2	daf-18(lf)	2.99±0.01	0.9	0.0	107
3	lin-3(lf)	0.81±0.19	95.2	0.0	21
4	daf-18(lf) lin-3(lf)	0.46±0.13	94.1	0.0	34
5	let-23(rf)	0.55±0.08	93.8	0.0	129
6	let-23(rf); daf-18(lf)	1.90±0.10*** (5)	63.9	6.8	133
7	lin-2(lf)	1.34±0.19	73.7	0.0	42
8	daf-18(lf); lin-2(lf)	2.50±0.12*** (7)	35.5	1.7	59
9	let-60(gf); sos-1(lf) #	2.60±0.17	16.7	0.0	30
10	daf-18(lf) let-60(gf); sos-1(lf) #	4.19±0.12*** (9)	0.0	90.3	31
11	lin-45(rf)	1.81±0.15	57.3	0.0	68
12	daf-18(lf) lin-45(rf)	2.29±0.17* (11)	39.5	2.3	42
13	mpk-1(lf)	0.00±0.00	100	0.0	14
14	mpk-1(lf); daf-18(lf)	0.00±0.00	100	0.0	17
15	let-60(gf) - gonad	2.44±0.27	46	23	13
16	daf-18(lf) let-60(gf) - gonad	3.38±0.38	8	42	12

SE indicates the standard error of the mean. % Vul indicates the fraction of animals with three or less induced VPCs. % Muv indicates the fraction of animals with more than three induced VPCs. Induction indicates the average number of induced VPCs per animal.

^*indicates a p-value<0.05.

^***indicates a p-value<0.0005, numbers in brackets indicates the row number against which a t-test was performed. Alleles used: LG II: let-23(sy1), LG III: mpk-1(ga117), LG IV: daf-18(ok480), let-60(n1046), lin-3(e1417), lin-45(sy96), LG V: sos-1(s1031), LG X: lin-2(n397).

^# sos-1(s1031) is cis-linked to unc-46(e177).