Table 1. Induction of golden mutant cells by the gol-ex2 TALEN.
Host embryo Genotype | Amount TALEN RNA injected | # embryos | # (%) embryos with golden cells |
golb1/+ | Uninjected | 37 | 0 |
100 pg | 20 | 19 (95.0%) | |
gol+/+ | Uninjected | 295 | 0 |
4 pg | 92 | 38 (41.3%) | |
20 pg | 79 | 71 (89.9%) | |
100 pg | 275 | 271 (98.5%) |
One cell stage embryos were injected with gol-ex2 TALEN mRNAs as indicated and the appearance of clones of pigmentless cells was scored in the 2 dpf RPE.